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Key Documents

AB5302

Sigma-Aldrich

Anti-Amyloid Precursor Protein Antibody, KPI Domain

serum, Chemicon®

Synonym(s):

APP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

monkey, mouse, human, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_000484.2
NM_201413.1
NM_201414.1

UniProt accession no.

P05067

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

Specificity

Amyloid Precursor Protein (APP), KPI Domain.

Immunogen

APP.

Application

Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.

Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.

Optimal working dilutions must be determined by end user.

Reduction /Alkylation protocol:

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vivo regulation of amyloid precursor protein neuronal splicing by microRNAs.
Smith P, Al Hashimi A, Girard J, Delay C, Hebert SS.
Journal of Neurochemistry null
Jingdong Zhang et al.
Journal of neuroinflammation, 18(1), 100-100 (2021-04-28)
Microglia are resident innate immune cells in the brain, and activation of these myeloid cells results in secretion of a variety of pro-inflammatory molecules, leading to the development of neurodegenerative disorders. Lipopolysaccharide (LPS) is a widely used experimental stimulant in
E Campbell et al.
Journal of neuroscience methods, 93(2), 133-138 (2000-01-14)
A novel polyclonal antibody (Ab993), specific for a KPI domain epitope of APP, was characterised for use in immunoprecipitation, Western blotting and immunohistochemistry. Conditioned medium from NTera2/D1 cells was used for immunoprecipitation and Western blots. Paraffin-embedded human brain sections were
Xinglong Yang et al.
Journal of biomedical research, 32(2), 136-144 (2018-01-24)
Microglia activation and white matter injury coexist after repeated episodes of mild brain trauma and ischemic stroke. Axon degeneration and demyelination can activate microglia; however, it is unclear whether early microglia activation can impair the function of white matter tracts

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