189485

Autocamtide-2 Related Inhibitory Peptide II, Cell-permeable

The Autocamtide-2 Related Inhibitory Peptide II, Cell-permeable controls the biological activity of calmodulin-dependent protein kinase II. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): Autocamtide-2 Related Inhibitory Peptide II, Cell-permeable, Ant-A3K/V10F-AIP, Ant-AIP-II, Ac-RQIKIWFQNRRMKWKKKKKLRRQEAFDAL-OH
• Empirical Formula (Hill Notation): C₁₇₇H₂₉₁N₅₇O₃₉S
• Molecular Weight: 3873.63
• UNSPSC Code: 12352202

Properties

• Quality Level: 200
• Assay: ≥97% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic); protect from light
• color: white
• solubility: 5% acetic acid: 1 mg/mL; water: 1 mg/mL; DMSO: 5 mg/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

A highly specific, potent, cell-permeable calmodulin-dependent protein kinase II (CaMKII) inhibitor that contains the Antennapedia transport peptide sequence fused to the amino terminus of AIP-II (Cat. No. 189484).

A highly specific, potent, cell-permeable calmodulin-dependent protein kinase II (CaMKII) inhibitory peptide that contains the Antennapedia transport peptide sequence fused to the N-terminus of autocamtide inhibitory peptide-II (AIP-II) (Cat. No. 189484). AIP-II is an AIP analog where Ala³ and Val¹⁰ are replaced by Lys and Phe residues. AIP-II is reported to be more potent than CaMK-(281-302/Ala²⁸⁶) and KN-93 (Cat. No. 422708). Inhibits by binding to the autophosphorylation site, which is distinct from the exogenous substrate-binding site.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
cam Kinase-2

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Lys-Lys-Lys-Leu-Arg-Arg-Gln-Glu-Ala-Phe-Asp-Ala-Leu-OH

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Other Notes

Watterson, D.M., et al. 2001. Neurochem. Int.39, 459.
Ishida, A., et al. 1998. FEBS Lett.427, 115.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable