1.50035

Purospher^®STAR RP-8 endcapped (5 µm) HPLC Columns

L × I.D. 250 mm x 4 mm Hibar^® RT HPLC Column

• Synonym(s): Purospher^® STAR RP-8 endcapped (5µm) Hibar^® RT 250-4
• UNSPSC Code: 41115709
• NACRES: SB.52

Properties

• product name: Purospher^® STAR RP-8 endcapped (5 µm), L × I.D. 250 mm x 4 mm Hibar^® RT HPLC column
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L7
• product line: Purospher^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 11.2% Carbon loading
• parameter: 400 bar pressure; 65 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 25 cm × 4 mm
• surface area: 330 m²/g
• matrix: fully porous particle
• matrix active group: C8 bonding phase
• particle size: 5 μm
• pore size: 120 Å pore size
• operating pH range: 1.5-10.5
• separation technique: reversed phase; supercritical fluid (SFC)
• storage temp.: no temp limit

Description

General description

Purospher^® STAR RP-8 endcapped, 5 µm HPLC columns allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range. Purospher^® STAR RP-8 endcapped, 5 µm Hibar^® RT 250-4 is a ready to use UHPLC column suitable for connection to any HPLC system.The column dimension of 250 mm length x 4 mm I.D. is the optimal choice for the HPLC separation of complex samples.Item 1.50035.1003 is a Validation kit containing 3 Purospher^® STAR RP-8 endcapped, 5 µm Hibar^® RT 250-4 columns from 3 different base material lots.Learn more about Purospher^® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, amino acids, esters, fatty acids, ketones, nitrosamines, nucleosides, PCBs, peptides, phenols, preservatives, steroids, and metabolized steroids.

Features and Benefits

• Ultra-pure silica (99.999%) for excellent peak symmetry
• High separation efficiency
• Reproducible results from run-to-run and batch-to-batch
• Outstanding pH stability from pH 1.5 to 10.5
• Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 125 bar
Theoretical Plates (N/m) (Toluene): ≥ 85000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.0

Legal Information

Hibar is a registered trademark of Merck KGaA, Darmstadt, Germany

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany