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14-736-M

Sigma-Aldrich

TAO2 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a MAP3K that doubly phosphorylates & activates the MAP2Ks, MEK3 & MEK6. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32

Pricing and availability is not currently available.

biological source

human

Quality Level

recombinant

expressed in Sf21 cells

Assay

84% (Coomassie blue staining, SDS-PAGE)

specific activity

1562 U/mg

mol wt

Mw 138.3 kDa

manufacturer/tradename

Chemicon®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

UniProt accession no.

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This Item
14-749-M14-739-M14-688-M
recombinant

expressed in Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

assay

84% (Coomassie blue staining, SDS-PAGE)

assay

93% (Coomassie blue staining, SDS-PAGE)

assay

-

assay

-

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

mol wt

Mw 138.3 kDa

mol wt

Mw 41 kDa

mol wt

Mw 48.8 kDa

mol wt

Mw 80 kDa

Gene Information

human ... TAOK2(9344)

Gene Information

human ... TAOK1(57551)

Gene Information

-

Gene Information

human ... PRKG1(5592)

General description

N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.
Product Source: expressed in Sf21 cells

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: TAO2
Target Sub-Family: STE

Quality

Routinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.

Unit Definition

Specific Activity: where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.

Physical form

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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