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usage
sufficient for 10 sample(s) (IgG-containing; 60-180 μl each)
sufficient for 20 sample(s) (Albumin-containing; 60-180 μl each)
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
protect from light
input
sample type serum
sample type plasma
sample type cerebrospinal fluid (CSF)
shipped in
wet ice
storage temp.
2-8°C
General description
High capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples, either on an LC-instrument or manually, attached to a syringe. Removal of these high abundance proteins allows enhanced detection of low abundance proteins.
Components
Two Albumin Removal Columns, one IgG Removal Column, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, IgG Elution Buffer, and a user protocol.
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Principle
The ProteoExtract Albumin/IgG Removal Kit, Maxi provides a convenient, reproducible and highly specific method to remove serum albumin and immunoglobulins from body fluid samples. The kit provides columns containing either a novel albumin-specific affinity resin or a proprietary immobilized protein A polymeric resin. These columns allow for the removal of more than 80% of both serum albumin and immunoglobulins from human body fluid samples when used according to the instructions provided in this manual. The column hardware is designed to allow for both manual and LC-instrument controlled usage. When used manually, the columns are operated as syringe-tip filters, when used with LC-instrument control, the columns can be connected to any standard HPLC/FPLC instrument via a 10/32" port using the provided adapters. For guidelines of column connections and operation, please refer to section 3 of this manual.
Preparation Note
The albumin removal column contains a resin that has been optimized for the removal of albumin from human samples. However, the provided columns will also bind albumins from other species although with lower efficiency. The dynamic binding capacity of one column was determined to be 6.1 ± 0.3 mg human albumin in Binding Buffer at a flow rate of 0.1 ml/min and 10 % breakthrough. To provide guidelines for different species we have evaluated the relative amount of albumin bound to the albumin removal column (Table 2).<div class="Bio_doc_image">
Table 2: Albumin Removal Column
</div>The Binding Buffer provided with the kit has been optimized to allow for specific binding of both albumin and immunoglobulins to the respective column without requirement of buffer exchange. This allows for the subsequent use of the two types of columns either independently using the flow through of the albumin removal column as sample load for the IgG removal column or with the two types of columns being directly connected in a row.The IgG Removal Column contains Protein A which is a highly stable surface receptor produced by Staphylococcus aureus. Protein A binds the Fc portion of IgGs from a large number of species. The dynamic binding capacity of the Protein A column was determined to be 4.5 mg ± 0.2 mg human IgG in Binding Buffer at a flow rate of 0.1 ml/min and 10 % breakthrough. The provided removal columns are guaranteed to be functional as indicated when bound proteins are eluted with the provided removal buffers and columns are used according to the manufacturers instructions given in this manual.
Table 2: Albumin Removal Column
</div>The Binding Buffer provided with the kit has been optimized to allow for specific binding of both albumin and immunoglobulins to the respective column without requirement of buffer exchange. This allows for the subsequent use of the two types of columns either independently using the flow through of the albumin removal column as sample load for the IgG removal column or with the two types of columns being directly connected in a row.The IgG Removal Column contains Protein A which is a highly stable surface receptor produced by Staphylococcus aureus. Protein A binds the Fc portion of IgGs from a large number of species. The dynamic binding capacity of the Protein A column was determined to be 4.5 mg ± 0.2 mg human IgG in Binding Buffer at a flow rate of 0.1 ml/min and 10 % breakthrough. The provided removal columns are guaranteed to be functional as indicated when bound proteins are eluted with the provided removal buffers and columns are used according to the manufacturers instructions given in this manual.
Storage and Stability
Upon arrival store entire contents of the kit at 4°C. Avoid freezing of the ProteoExtract Blue and ProteoExtract Protein A columns! Freezing thawing will destroy the affinity resin and the columns no longer function.
Other Notes
Boyle, M. D. P. and K. J. Reis., 1987. Biotechnology5, 697.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PROTEOEXTRACT is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
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