1.12374
Thrombin
from bovine plasma, 50 NIH units/mg dry wt
Synonym(s):
Thrombin, Coagulation factor IIa, Fibrinogenase
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About This Item
Recommended Products
biological source
bovine plasma
Quality Level
form
solid
specific activity
50 NIH units/mg dry wt
storage temp.
2-8°C
General description
Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.
Application
Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.
Biochem/physiol Actions
Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.
Analysis Note
Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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