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HomeMetabolomics ResearchChemical Compatibility Guide for MultiScreen® Filter Plates

Chemical Compatibility Guide for MultiScreen® Filter Plates

Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for short exposures, but this must be determined on an individual assay basis.

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Plate Material/MembranePolyolefin
copolymer/PTFE
Acrylic or Classic
Styrene/Durapore®
Acrylic or Styrene/ MCEBarex®/TiO2/
Durapore®, MCE, DEAE
Acrylic or Barex®/
TiO2/Styrene,
Immobilon®-P
Acids
Acetic (5%)RRRRR
Acetic, GlacialRL to NRNRR/L to NRL
BoricRRRRR
Trichloroacetic (<20%)RRRRR
Trichloroacetic (20 to 40%)RRLR/L to NRL
Trichloroacetic (>40%)RL to NRNRL/NRNR
Hydrochloric (0.1 N)RRRRR
Hydrochloric (1 N)RRL to NRR/L to NRR to L
Hydrochloric (>1 N)RRNRR/L to NRR
HydrofluoricRR (20% max.)NRNRR (20% max.)
Nitric (conc)RL to NRNRNRL
Sulfuric (conc)RLNRL/NRL
Bases
NH4OH (6 N)RL to NRNRL to NRR
NaOH (0.1 N)RR to LL to NRR/L to NRR
NaOH (1 N)RL to NRNRL/NRL
NaOH (>6 N)RNRNRNRNR
UreaRRR (Durapore® only)R
TriethylamineRRRRR
Diethanolamine (≤3 mol)RRRRR
Alcohols
AmylRRNRR/NRR
Benzyl (1%)RRRRR
ButylRRRRR
Ethanol (40%)RRNRRR
Ethanol (≥50%)RRNRR/NRR (no filtrate collection)
IsobutylRRRRR
IsopropylRRL to NRR/L to NRR
MethanolRR to LNRR/NRR
Triton® X-100 Surfactant (<1%)RRRRR
R = Recommended, no known restrictions, NR = Not recommended, membrane or plate severely attacked by chemical, L = Limited, chemical resistance marginal, short time exposures should be tested individually for application. Dilution with water or other non-solvent will likely enhance compatibility.
  1. Scintillants should only be used with Barex® or SAN plates.
  2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Plate Material/MembranePolyolefin
copolymer/PTFE
Acrylic or Classic
Styrene/Durapore®
Acrylic or Styrene/ MCEBarex®/TiO2/
Durapore®, MCE, DEAE
Acrylic or Barex®/
TiO2/Styrene,
Immobilon®-P
Solvents
AcetoneRNRNRNRNR
Acetonitrile (no filtrate collection, centrifuge)RNRNRNRL (<35%)
Amyl AcetateRNRNRR/NRR
Carbon TetrachlorideRL to NRL to NRRR
ChloroformRNRNRNRNR
CyclohexanoneRNR to LNR to LR/ NR to LNR to L
DMACRNRNRNRNR
DMFRNRNRNRNR
DMSO (no filtrate collection)RL (70%)NRNRL (10%) max.
Ethylene GlycolRRRRR
FormaldehydeRR (10% max.)NRR (<40%)/NRR (<40%)
HexaneNRRRRR
Methylene ChlorideLNRNRNRNR
MEKRNRNRNRNR
MIBKRNRNRL/NRNR/L
Phenol (5%)RL to NRL to NRRR
PyridineRRRRR
ScintillantsNRNRNRRNR/R(1)
TriethylamineRRRRR
TolueneNRNR to LNR to LRNR to L/R
THFRNRNRL/NRNR/L
XyleneRNRNRRR
Other Organics
Attophos® ReagentRRRRR
FluoresceinRRRRR
GlycerineRRRRR
Hydrogen PeroxideRRNRR (3%)R
Polyethylene GlycolRRRRR
Tween® Surfactant (<5%) (2)RRRRR
Triton® X-100 Surfactant (<1%)RRRRR
Triton® X-100 Surfactant (<5%)RRLR/LR
Inorganic Salts
Carbon DisulfiteRNRNRNRNR
Sodium HypochloriteRRNRR (5%)/ NRR
R = Recommended, no known restrictions, NR = Not recommended, membrane or plate severely attacked by chemical, L = Limited, chemical resistance marginal, short time exposures should be tested individually for application. Dilution with water or other non-solvent will likely enhance compatibility.
  1. Scintillants should only be used with Barex® or SAN plates.
  2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

Chemical Compatibility Guide for the MultiScreen® Vacuum Manifolds

MultiScreen®HTS and MultiScreen® Resist Manifolds

The following tables outline the solvents that have been evaluated for compatibility with the various components of the MultiScreen®HTS vacuum manifold. In general, the manifold will tolerate the same chemicals as the rest of the system.

However, when using concentrated acids, it is important to rinse through the entire manifold at the end of procedures, especially if collection is done through the manifold into a trap, rather than using the collection trays.

MultiScreen®HTS Vacuum Manifold Components

ComponentManifold Base/
Collar Gasket
Frame
Gaskets/TubingStandard CollarSupport GridTubing FittingsDroplet Trap
Array
Materials of ConstructionHDPE/
Polypropylene,
Polyolefin
SiliconeNylonStainless SteelPP with EPDM or
Fluoroelastomer
Seals
PTFE
AcetoneRGGEGE
AcetonitrileEGEEGE
Dimethyl Formamide (DMF)EGREGE
Dimethyl Sulfoxide (DMSO)EGEEGE
Ethyl AcetateEGEEGE
EthanolEGGEEE
Formic AcidEGNRGGR
HexaneNRNRREGE
Hydrochloric Acid (37%)ERNRRRR
IsopropanolEEREEE
MethanolEEREEE
Methylene ChlorideNRNRRERE
Sodium HypochloriteEGNRGGR
Tetrahydrofuran (THF)RNREENRE
TolueneNRNREERE
Trichloroacetic Acid (TCA)ENRGGRR
Trifluoroacetic Acid (TFA)ENRRRGR
E = Excellent performance, G = Good performance, R = Rinse after contact, NR = Not recommended

MultiScreen® Resist Vacuum Manifold Components

ComponentManifold Base
& Deep Well Ring
GasketsStandard RingTubing Inside: OutsideSupport GridControl ValveOn/Off Valve
Materials of ConstructionHDPEEPDMNylonFEP-lined TygonStainless Steel  Brass socket
steel case
PP with EPDM seal
Comments   Crimping can alter resistance Normally no fluid contact 
AcetoneR-GGG-EE: NREEG-E
AcetonitrileEEEE: NREEG-E
Dimethyl Formamide (DMF)EERE: NREEG
Dimethyl Sulfoxide (DMSO)EEEE: REEG
Ethyl AcetateEEEE: NREGG-E
EthanolEEGE: EEEE
Formic AcidEENRG-E: NRG-EP-RG-E
HexaneRRRR-G: PEEG-E
Hydrochloric AcidEER-NRE: R   
IsopropanolEERE: GEEE
MethanolEERE: GEEE
Methylene ChlorideRRR-GE: NREEP-R
Sodium HypochloriteEENR-PE: RGGG
Tetrahydrofuran (THF)RREE: GEENR
TolueneRREE: REER
Trichloroacetic Acid (TCA)EEGE: GG-ERR-G
Trifluoroacetic Acid (TFA)EERE: RR-GP-RG
E = Excellent performance, G = Good performance, R = Rinse after contact, NR = Not recommended

Chemical Compatibility Guide for Fixation and Staining

Millicell® cell culture inserts (single-well, 24-well plates and 96-well plates) are designed to support all fixation, staining and immunostaining procedures in a single device. The 24 and 96-well plates are also automation compatible. The variety of membranes offered in the inserts allows the fixed and stained cells to be visualized by stereoscopic microscopy, phase contrast microscopy, or fluorescent methods.

The majority of staining procedures employ a fixation step first. Fixation is required to stabilize sub-cellular morphology and prevent degradation of antigens during subsequent staining procedures. Consult the following table for chemical compatibility information with common fixative chemicals and stains.

General Considerations

  • If the chemicals are compatible with the membrane but not the polystyrene housing, remove the membrane from the housing before adding the chemical.
  • Unless otherwise stated, the chemicals listed are at maximum concentration. If the plastic housing and/or membranes are not compatible with the maximum concentration, they might be compatible at lower concentration.

Chemical Compatibilty of Fixation and Staining Reagents

ChemicalConcentrationPolystyrene (PS) housingHA (Mixed Cellulose) membraneCM (PTFE)PCF
(Polycarbonate)
  PET (polyteterep- thalate)
Methanol90%RNRRRR
Ethanol95%RRRRR
Glacial Acetic Acid1%RRRRR
Paraformaldehyde4%RRRRR
Glutaraldehyde3%RRRRR
Formaldehyde4%RRRRR
Formamide50%RNRRRR
Formamide100%RNRRRR
HCL1.0%RRRRR
Osmium tetroxide1.0%RRRRR
Hematoxylin100%RRRRR
Eosin1.0%RRRRR
Tryptan Blue0.1%RRRRR
Nigrosin0.2%RRRRR
Toludine Blue0.3%RRRRR
R = recommended, NR = not recommended
Materials
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