Chemical Compatibility Guide for MultiScreen® Filter Plates
Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for short exposures, but this must be determined on an individual assay basis.
Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents | |||||
---|---|---|---|---|---|
Plate Material/Membrane | Polyolefin copolymer/PTFE | Acrylic or Classic Styrene/Durapore® | Acrylic or Styrene/ MCE | Barex®/TiO2/ Durapore®, MCE, DEAE | Acrylic or Barex®/ TiO2/Styrene, Immobilon®-P |
Acids | |||||
Acetic (5%) | R | R | R | R | R |
Acetic, Glacial | R | L to NR | NR | R/L to NR | L |
Boric | R | R | R | R | R |
Trichloroacetic (<20%) | R | R | R | R | R |
Trichloroacetic (20 to 40%) | R | R | L | R/L to NR | L |
Trichloroacetic (>40%) | R | L to NR | NR | L/NR | NR |
Hydrochloric (0.1 N) | R | R | R | R | R |
Hydrochloric (1 N) | R | R | L to NR | R/L to NR | R to L |
Hydrochloric (>1 N) | R | R | NR | R/L to NR | R |
Hydrofluoric | R | R (20% max.) | NR | NR | R (20% max.) |
Nitric (conc) | R | L to NR | NR | NR | L |
Sulfuric (conc) | R | L | NR | L/NR | L |
Bases | |||||
NH4OH (6 N) | R | L to NR | NR | L to NR | R |
NaOH (0.1 N) | R | R to L | L to NR | R/L to NR | R |
NaOH (1 N) | R | L to NR | NR | L/NR | L |
NaOH (>6 N) | R | NR | NR | NR | NR |
Urea | R | R | — | R (Durapore® only) | R |
Triethylamine | R | R | R | R | R |
Diethanolamine (≤3 mol) | R | R | R | R | R |
Alcohols | |||||
Amyl | R | R | NR | R/NR | R |
Benzyl (1%) | R | R | R | R | R |
Butyl | R | R | R | R | R |
Ethanol (40%) | R | R | NR | R | R |
Ethanol (≥50%) | R | R | NR | R/NR | R (no filtrate collection) |
Isobutyl | R | R | R | R | R |
Isopropyl | R | R | L to NR | R/L to NR | R |
Methanol | R | R to L | NR | R/NR | R |
Triton® X-100 Surfactant (<1%) | R | R | R | R | R |
- Scintillants should only be used with Barex® or SAN plates.
- Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).
Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents | |||||
---|---|---|---|---|---|
Plate Material/Membrane | Polyolefin copolymer/PTFE | Acrylic or Classic Styrene/Durapore® | Acrylic or Styrene/ MCE | Barex®/TiO2/ Durapore®, MCE, DEAE | Acrylic or Barex®/ TiO2/Styrene, Immobilon®-P |
Solvents | |||||
Acetone | R | NR | NR | NR | NR |
Acetonitrile (no filtrate collection, centrifuge) | R | NR | NR | NR | L (<35%) |
Amyl Acetate | R | NR | NR | R/NR | R |
Carbon Tetrachloride | R | L to NR | L to NR | R | R |
Chloroform | R | NR | NR | NR | NR |
Cyclohexanone | R | NR to L | NR to L | R/ NR to L | NR to L |
DMAC | R | NR | NR | NR | NR |
DMF | R | NR | NR | NR | NR |
DMSO (no filtrate collection) | R | L (70%) | NR | NR | L (10%) max. |
Ethylene Glycol | R | R | R | R | R |
Formaldehyde | R | R (10% max.) | NR | R (<40%)/NR | R (<40%) |
Hexane | NR | R | R | R | R |
Methylene Chloride | L | NR | NR | NR | NR |
MEK | R | NR | NR | NR | NR |
MIBK | R | NR | NR | L/NR | NR/L |
Phenol (5%) | R | L to NR | L to NR | R | R |
Pyridine | R | R | R | R | R |
Scintillants | NR | NR | NR | R | NR/R(1) |
Triethylamine | R | R | R | R | R |
Toluene | NR | NR to L | NR to L | R | NR to L/R |
THF | R | NR | NR | L/NR | NR/L |
Xylene | R | NR | NR | R | R |
Other Organics | |||||
Attophos® Reagent | R | R | R | R | R |
Fluorescein | R | R | R | R | R |
Glycerine | R | R | R | R | R |
Hydrogen Peroxide | R | R | NR | R (3%) | R |
Polyethylene Glycol | R | R | R | R | R |
Tween® Surfactant (<5%) (2) | R | R | R | R | R |
Triton® X-100 Surfactant (<1%) | R | R | R | R | R |
Triton® X-100 Surfactant (<5%) | R | R | L | R/L | R |
Inorganic Salts | |||||
Carbon Disulfite | R | NR | NR | NR | NR |
Sodium Hypochlorite | R | R | NR | R (5%)/ NR | R |
- Scintillants should only be used with Barex® or SAN plates.
- Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).
Chemical Compatibility Guide for the MultiScreen® Vacuum Manifolds
MultiScreen®HTS and MultiScreen® Resist Manifolds
The following tables outline the solvents that have been evaluated for compatibility with the various components of the MultiScreen®HTS vacuum manifold. In general, the manifold will tolerate the same chemicals as the rest of the system.
However, when using concentrated acids, it is important to rinse through the entire manifold at the end of procedures, especially if collection is done through the manifold into a trap, rather than using the collection trays.
MultiScreen®HTS Vacuum Manifold Components | ||||||
---|---|---|---|---|---|---|
Component | Manifold Base/ Collar Gasket Frame | Gaskets/Tubing | Standard Collar | Support Grid | Tubing Fittings | Droplet Trap Array |
Materials of Construction | HDPE/ Polypropylene, Polyolefin | Silicone | Nylon | Stainless Steel | PP with EPDM or Fluoroelastomer Seals | PTFE |
Acetone | R | G | G | E | G | E |
Acetonitrile | E | G | E | E | G | E |
Dimethyl Formamide (DMF) | E | G | R | E | G | E |
Dimethyl Sulfoxide (DMSO) | E | G | E | E | G | E |
Ethyl Acetate | E | G | E | E | G | E |
Ethanol | E | G | G | E | E | E |
Formic Acid | E | G | NR | G | G | R |
Hexane | NR | NR | R | E | G | E |
Hydrochloric Acid (37%) | E | R | NR | R | R | R |
Isopropanol | E | E | R | E | E | E |
Methanol | E | E | R | E | E | E |
Methylene Chloride | NR | NR | R | E | R | E |
Sodium Hypochlorite | E | G | NR | G | G | R |
Tetrahydrofuran (THF) | R | NR | E | E | NR | E |
Toluene | NR | NR | E | E | R | E |
Trichloroacetic Acid (TCA) | E | NR | G | G | R | R |
Trifluoroacetic Acid (TFA) | E | NR | R | R | G | R |
MultiScreen® Resist Vacuum Manifold Components | |||||||
---|---|---|---|---|---|---|---|
Component | Manifold Base & Deep Well Ring | Gaskets | Standard Ring | Tubing Inside: Outside | Support Grid | Control Valve | On/Off Valve |
Materials of Construction | HDPE | EPDM | Nylon | FEP-lined Tygon | Stainless Steel | Brass socket steel case | PP with EPDM seal |
Comments | Crimping can alter resistance | Normally no fluid contact | |||||
Acetone | R-G | G | G-E | E: NR | E | E | G-E |
Acetonitrile | E | E | E | E: NR | E | E | G-E |
Dimethyl Formamide (DMF) | E | E | R | E: NR | E | E | G |
Dimethyl Sulfoxide (DMSO) | E | E | E | E: R | E | E | G |
Ethyl Acetate | E | E | E | E: NR | E | G | G-E |
Ethanol | E | E | G | E: E | E | E | E |
Formic Acid | E | E | NR | G-E: NR | G-E | P-R | G-E |
Hexane | R | R | R | R-G: P | E | E | G-E |
Hydrochloric Acid | E | E | R-NR | E: R | |||
Isopropanol | E | E | R | E: G | E | E | E |
Methanol | E | E | R | E: G | E | E | E |
Methylene Chloride | R | R | R-G | E: NR | E | E | P-R |
Sodium Hypochlorite | E | E | NR-P | E: R | G | G | G |
Tetrahydrofuran (THF) | R | R | E | E: G | E | E | NR |
Toluene | R | R | E | E: R | E | E | R |
Trichloroacetic Acid (TCA) | E | E | G | E: G | G-E | R | R-G |
Trifluoroacetic Acid (TFA) | E | E | R | E: R | R-G | P-R | G |
Chemical Compatibility Guide for Fixation and Staining
Millicell® cell culture inserts (single-well, 24-well plates and 96-well plates) are designed to support all fixation, staining and immunostaining procedures in a single device. The 24 and 96-well plates are also automation compatible. The variety of membranes offered in the inserts allows the fixed and stained cells to be visualized by stereoscopic microscopy, phase contrast microscopy, or fluorescent methods.
The majority of staining procedures employ a fixation step first. Fixation is required to stabilize sub-cellular morphology and prevent degradation of antigens during subsequent staining procedures. Consult the following table for chemical compatibility information with common fixative chemicals and stains.
General Considerations
- If the chemicals are compatible with the membrane but not the polystyrene housing, remove the membrane from the housing before adding the chemical.
- Unless otherwise stated, the chemicals listed are at maximum concentration. If the plastic housing and/or membranes are not compatible with the maximum concentration, they might be compatible at lower concentration.
Chemical Compatibilty of Fixation and Staining Reagents | ||||||
---|---|---|---|---|---|---|
Chemical | Concentration | Polystyrene (PS) housing | HA (Mixed Cellulose) membrane | CM (PTFE) | PCF (Polycarbonate) | PET (polyteterep- thalate) |
Methanol | 90% | R | NR | R | R | R |
Ethanol | 95% | R | R | R | R | R |
Glacial Acetic Acid | 1% | R | R | R | R | R |
Paraformaldehyde | 4% | R | R | R | R | R |
Glutaraldehyde | 3% | R | R | R | R | R |
Formaldehyde | 4% | R | R | R | R | R |
Formamide | 50% | R | NR | R | R | R |
Formamide | 100% | R | NR | R | R | R |
HCL | 1.0% | R | R | R | R | R |
Osmium tetroxide | 1.0% | R | R | R | R | R |
Hematoxylin | 100% | R | R | R | R | R |
Eosin | 1.0% | R | R | R | R | R |
Tryptan Blue | 0.1% | R | R | R | R | R |
Nigrosin | 0.2% | R | R | R | R | R |
Toludine Blue | 0.3% | R | R | R | R | R |
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