Solid Phase Microextraction (SPME)
Solid phase microextraction (SPME) is an innovative and sensitive solvent-free sample preparation technology. Based on the principle of adsorption/absorption and desorption, SPME uses a coated fiber to concentrate volatile and semi-volatile compounds from a sample.
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SPME is widely used for a variety of applications involving environmental, biological and pharmaceutical samples, foods and beverages, flavors and fragrances, forensics and toxicology and product testing. Typical uses include:
- Environmental analyses of water & air samples
- Headspace analysis of trace impurities in polymers and solid samples
- Part-per-trillion odor analyses
- Flavor analyses of food products
- Forensic analysis of arson and explosives samples
- Toxicology analyses of blood alcohol or drugs in urine and serum
How Does SPME Work?
SPME uses a fiber coated with an extraction phase: a liquid (polymer), a solid (sorbent), or a combination of both. The coated fiber is housed in a protective needle and attached to a holder that looks like a syringe.
When the fiber is exposed to a sample, the sample’s analytes partition from the sample matrix into the stationary phase until an equilibrium is established. The fiber’s coating extracts compounds from the sample either by absorption (liquid coatings) or adsorption (solid coatings). After a prescribed extraction time, the fiber is removed and inserted directly into a chromatographic instrument, usually gas chromatography (GC) or HPLC, for desorption and analysis. The desorption in GC of analytes is carried out thermally, whereas HPLC uses a solvent for desorption into a liquid-phase.
Advantages of SPME
SPME combines analyte sampling, isolation, and enrichment into one, simple step. By controlling the polarity and thickness of the fiber coating, maintaining consistent sampling time, and controlling several other extraction parameters, SPME allows an analyst to ensure highly consistent and quantifiable results from samples, even when analytes are at low concentrations.
Other benefits of SPME include:
- Solvent-free
- Easy to automate
- Non-destructive to samples
- Applicable for nearly any sample or matrix
- Fibers used are reusable and inexpensive
- Small fiber size makes them agreeable to field work
- Compatible with GC or HPLC instrumentation
SPME Fibers for GC Analysis
Conventional SPME is used to extract and concentrate analytes for the purpose of GC analysis. Extraction is carried out either by direct immersion (DI-SPME), where the fiber is directly immersed in the liquid sample, or headspace SPME (HS-SPME), where the fiber is exposed in the vapor phase above a sample.
Supel™ BioSPME for LC-MS Analysis
Supel™ BioSPME is a bioanalytical microsampling and sample preparation technique used to quickly and selectively extract a broad range of analytes from biological samples while repelling unwanted macromolecules (e.g., lipids, proteins). Subsequent analysis is usually performed by LC-MS. Supel™ BioSPME operates via direct extraction, involving no sample pretreatment, and provides a non-exhaustive, equilibrium-based extraction.
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Related Protocols
- Aldehydes and ketones are ubiquitous air pollutants. Along with esters and ethers, they are major components of indoor air pollution and are therefore important for industrial hygiene applications.
- Separation of 2-Ethyl-3-methylpyrazine; 1-Methylpyrrole; 2,3-Dimethylpyrazine; 2,5-Dimethylpyrazine; 2-Ethylpyrazine, ≥98%, FG; 2,3-Diethylpyrazine; 2-Methylpyrazine; Carbon disulfide; Dimethyl disulfide; 2,6-Dimethylpyrazine
- Bisphenol A (BPA) is suspected to be an endocrine disruptor. Regulations on specific migration limits (SML) from food contact materials are getting tighter (EU 2018/213) requiring more sensitive determinations. An overcoated SPME fiber provides the durability necessary for direct immersion into foods such as canned soup and pumpkin, allowing for low level analysis of BPA via SPME-GC-MS/MS.
- High performance, reliability, and reproducibility of HS-SPME in combination with GC/MS for the determination of VOCs in water was proven in an interlaboratory trial. The new ISO 17943 using HS-SPME is an improvement on existing official methods for this determination in terms of sensitivity and selectivity.
- Separation of 2-Isopropyl-3-methoxypyrazine 20 ppt; 2-Isobutyl-3-methoxypyrazine; 2-Methylisoborneol; (±)-Geosmin 10 ppt
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