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LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide

LC/MS Analysis of Methadone and Metabolites EDDP and EMDP on Ascentis® Express RP Amide application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53913-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

M-007

(±)-Methadone solution

1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

E-022

EDDP perchlorate solution

1.0 mg/mL in methanol (as pyrolinium), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express RP Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)

mobile phase

[A] 10 mM ammonium formate in water, pH 3.6; [B] 10 mM ammonium formate in acetonitrile, pH 3.6; (65:35, A:B)

flow rate

0.5 mL/min

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

0.5 μL

sample

200 μg/L in 25:75, water: acetonitrile

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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