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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Supelco
Methanol solution, contains 0.10 % (v/v) formic acid, UHPLC, suitable for mass spectrometry (MS), ≥99.5%
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Residual Solvent - Chloroform, Pharmaceutical Secondary Standard; Certified Reference Material
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Ammonium hydroxide solution, BioUltra, ~1 M NH3 in H2O (T)
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4-Methylmorpholine, BioXtra, suitable for protein sequencing, ≥99.5% (GC)
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Toluene, anhydrous, 99.8%
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Pyridine, anhydrous, 99.8%
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
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Pyridine, ≥99%
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Toluene, analytical standard
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Triphenyl phosphate, analytical standard
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Methanol-12C, 99.95 atom % 12C
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Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D)
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Toluene, Laboratory Reagent, ≥99.3%
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Ammonium hydroxide solution, puriss. p.a. plus, ≥25% NH3 in H2O
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Ammonium hydroxide solution, puriss. p.a., reag. ISO, reag. Ph. Eur., ~25% NH3 basis
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Ammonium hydroxide solution, ACS reagent, 28.0-30.0% NH3 basis
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Toluene, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.7% (GC)
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Ammonium hydroxide solution, 28% NH3 in H2O, ≥99.99% trace metals basis
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Pyridine, ReagentPlus®, ≥99%
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Toluene, ACS reagent, ≥99.5%
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Pyridine, biotech. grade, ≥99.9%
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Ammonium hydroxide solution, puriss., 30-33% NH3 in H2O
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Ammonium hydroxide solution, puriss., meets analytical specification of Ph. Eur., 25-30% NH3 basis
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Toluene, Pharmaceutical Secondary Standard; Certified Reference Material
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Calcium chloride, Vetec, reagent grade, 96%
USP
Methyl alcohol, United States Pharmacopeia (USP) Reference Standard
Benzyl alcohol, European Pharmacopoeia (EP) Reference Standard
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Pyridine, suitable for HPLC, ≥99.9%
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Toluene, ACS reagent, ≥99.5%
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Toluene, suitable for HPLC, 99.9%