Gel electrophoresis

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Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.

mPAGE® Lux Casting System for SDS-PAGE Gels

Discover the mPAGE® Lux Casting System for ready-to-use SDS-PAGE gels in 3 minutes. Fresh SDS-PAGE gels that are ready when you need them.

Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels

This page shows how to apply equilibrated Immobiline DryStrip gels

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Sample Preparation & Gel Electrophoresis Troubleshooting

The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Auto2D® Automated 2-D Gel Electrophoresis Device

The Auto2D® 2-D Electrophoresis Device fully automates difficult 2D electrophoresis methods in a quick, easy, and reproducible way. Locate difficult-to-find proteins using the Auto2D® system in less than two hours with high reproducibility.

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Proteomic analysis of membrane proteins

This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.

Auto2D® Automated 2-D Gel Electrophoresis Device Protocol

The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.

DNA / Protein Electrophoresis and Troubleshooting Tables

Gel Electrophoresis

Protein gel electrophoresis is used to separate proteins for purification, characterization, and detection. Methods such as native PAGE, SDS-PAGE, 2D PAGE, and isoelectric focusing (IEF) are used in preparation for downstream applications including Western blotting, mass spectrometry, and proteomic analysis.

Precast Agarose Gels for RNA Electrophoresis

Precast Agarose Gels for RNA Electrophoresis are suitable for separating RNA sizes from 0.25 to 10 kb.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System

This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.

Setting Up and Running TruPAGE™ Precast Gels

TruPAGE™ precast gels offer high rigidity for tear resistance and improved protein resolution so researchers can achieve quality separation the first time around.

Cleaning Up Samples using 2-D Clean-Up Kit

2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.

Two-Dimensional Separation of Protein Samples

This page describes two-dimensional separation of protein samples with GE Healthcare products.

Nucleic Acid Electrophoresis Protocols & Introduction

An introduction to Nucleric Acid electrophoresis and standard protocols for Agarose gel electrophoresis for DNA, RNA & polyacrylamide gel electrophoresis for DNA.

Rehydration Solutions

Selecting Markers and Ladders for Gel Electrophoresis

Sigma-Aldrich offers a variety of markers for nucleic acid size determination of sample separated by various gel electrophoresis methods.

Detergent Properties and Applications

Use detergents more effectively in cell lysis, membrane protein and lipid solubilization, protein crystallization, or Western blotting.

Troubleshooting First-Dimension IEF: MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit

This page describes troubleshooting first-dimension isoelectric focusing using MultiPhor™ II Electrophoresis System and Immobiline® DryStrip Kit.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System

Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.

Troubleshooting Second-Dimension SDS-PAGE: Multiphor™ II Electrophoresis

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using mPAGE® TurboMix Bis-Tris Gel Casting Kits.

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