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Key Documents

796-0658

HPLC Analysis of Proteins on Discovery® BIO PolyMA-SCX (Analyte Set #2)

application for HPLC

Synonym(s):

Proteins, mixture

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About This Item

description

Separated on Discovery® BIO PolyMA-SCX

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM sodium phosphate, pH 7.0; [B] 20 mM sodium phosphate, 0.5 M NaCl, pH 7.0; 5 to 100% B in 15 min. (linear)
flow rate: 0.5 mL/min
injection: 10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
detector: UV, 280 nm

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the power of ion-exchange to resolve proteins with very little difference in molecular weight, an advantage over size-exclusion separations.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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