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GE17-3712-20

HIS Mag Sepharose™ Excel

Cytiva 17-3712-20, pack of 2 × 1 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

IMAC immobilized with Ni

packaging

pack of 2 × 1 mL

manufacturer/tradename

Cytiva 17-3712-20

parameter

Ambient temp. range

matrix

highly cross-linked spherical agarose including magnetite

General description

His Mag Sepharose Excel are magnetic beads designed for rapid, small-scale purification and screening of his-tagged proteins consisting of an Immobilized Metal Affinity Chromatography (IMAC) resin precharged with nickel ions that are strongly bound to a chelating ligand.

Small-scale capture, purification, and screening of histidine-tagged proteins that are expressed and secreted into supernatants from eukaryotic cells such as insect cells or Chinese Hamster Ovary (CHO) cells in HyClone HyCell CHO cell culture media.

The base of the matrix is based on Sepharose resin, incorporated with magnetite. His Mag Sepharose is available as 10% slurry in three different pack sizes: 2 x 1 mL, 5 x 1 mL, and 10 x 1mL.

Compatible with MagRack 6 and MagRack Maxi, separation tools for handling the beads in microcentrifuge tubes.

Our ISO 13485 accredited manufacturing center of excellence adheres to the standards in the manufacturing process, ensuring the highest quality and compliance with international medical device regulations.

Features and Benefits

  • Simplify small-scale purification and expression screening of histidine-tagged proteins secreted in eukaryotic cell culture
  • Purify histidine-tagged proteins directly from samples that contain metal ion-stripping agents and save time and effort
  • Increase target protein yield and decrease degradation through reduced and simplified sample handling
  • Screen histidine-tagged proteins in parallel with high reproducibility and reliable results
  • Capture histidine-tagged protein from different sample volumes; from low to high milliliter scale
  • Simplify handling using visible and dense Sepharose-based magnetic beads

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% ethanol, 5% medium slurry)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Environment
GHS09

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using Cytiva products.

Manual and Automated Purification

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

Protocols

Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni

This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from GE Healthcare.

Purification of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service