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Z734438

ThermalSeal RTS™ Sealing Films

for qPCR, storage & crystallization, non-sterile

Synonym(s):

qPCR plate seal, storage plate seal

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About This Item

UNSPSC Code:
41122410
NACRES:
NB.22

material

polyolefin film
silicone/adhesive

sterility

non-sterile

packaging

pack of 100 ea

manufacturer/tradename

Excel Scientific TSS-RTQ-100

technique(s)

PCR: suitable
qPCR: suitable

L × W

76.2 mm × 133.4 mm

W × L

7.62 cm × 13.34 cm

color

clear

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General description

ThermalSeal RTS™ sealing film is a 50 μm thick polyolefin with 50 μm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert.

ThermalSeal RTS™ films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim.

Dimensions 76.2 by 133.4 mm. With end tabs removed, length is 113.0 mm.

  • High optical clarity
  • Minimal to no autofluorescence
  • Chemically inert; no extractables except at extreme pH
  • DMSO resistant for HTS
  • Heat resistant, recommended for temperatures from
  • -70°C to +100°C
  • Certified DNase, RNase, and Nucleic Acid free
  • Fit within raised plate rim to prevent loss of seal due to film lifting
  • Silicone adhesive forms the strongest available seal for evaporation prevention
  • Non-tacky adhesive layer simplifies handling of film prior to sealing

Legal Information

ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RTS is a trademark of Excel Scientific, Inc.

Certificates of Analysis (COA)

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Protocols

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Standard Reverse Transcription Protocol (Two-step)

Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.

Reverse Transcription Protocol Using SYBR Green Dye Detection

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

qPCR Using a Single Detection Probe Protocol

A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt® ReadyMix™.

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