14-782-M
PI 3-Kinase (p110α(E542K)/p85α), Human, 10 µg
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
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About This Item
UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
Recommended Products
biological source
human
Quality Level
100
300
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 125.3 kDa (p110α; E542K)
Mw 83.7 kDa (p85α)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Gene Information
human ... PIK3CA(5290)
General description
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K and untagged, recombinant, full-length human p85α.
The E542K substitution is a somatic mutation in p110α that has been associated with tumours of the colon and brain. Combined in vitro and in vivo studies have shown that this mutation confers higher lipid kinase activity than wild type, and is able to induce oncogenic transformation.
Application
Complex of N-terminal 6His-tagged, recombinant, full-length human p110α containing the mutation E542K & untagged, recombinant, full-length human p85α. For use in Kinase Assays.
Packaging
Also available in 250 μg size --call for pricing and availability and reference catalog number 14-782M when ordering the 250 μg size.
Storage and Stability
Store at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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