04-820
Anti-Estrogen Receptor α Antibody, clone 60C, rabbit monoclonal
culture supernatant, clone 60C, from rabbit
Synonym(s):
Estradiol receptor, Nuclear receptor subfamily 3 group A member 1, estrogen receptor 1, estrogen receptor alpha, oestrogen receptor, steroid hormone receptor
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
60C, monoclonal
species reactivity
rat, pig, mouse, human, bovine
packaging
antibody small pack of 25 μL
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
bovine ... Esr1(407238)
human ... ESR1(2099)
mouse ... Esr1(13982)
pig ... Esr1(397435)
rat ... Esr1(24890)
General description
The Estrogen Receptors (ER) belong to the nuclear receptor family that contains a ligand-binding domain that maintains the protein in an inactive conformation unless bound to the hormone. Two ERs are known, ERα, classically known as ER, and ERβ, described long after ERα. Activated Estrogen Receptor is a DNA-binding protein capable of inducing many changes in gene expression. The Estrogen Receptor is believed to play an important role in the growth and development of a subset of hormone dependent human breast cancers. ERβ is expressed in prostate, ovary, and other reproductive organs. Although its steroid specificity is very similar to that of the ERα, the molecular mechanisms regulating the transcriptional activity of ERβ may be distinct from those regulating ERα.
Specificity
Predicted to cross-react with rat, mouse, bovine and porcine based on sequence homology.
Recognizes Estrogen Receptor α. No cross-reactivity with Estrogen Receptor β.
Immunogen
Epitope: N-term
KLH-conjugated, synthetic peptide corresponding to amino acids 13-32 (C-ALLHQIQGNELEPLNRPQLK) of human Estrogen Receptor α. The immunizing sequence is identical in rat and mouse and has 19/20 identical amino acids in bovine and porcine.
Application
Anti-Estrogen Receptor α Antibody, clone 60C is an antibody against Estrogen Receptor α for use in WB & IP.
Immunoprecipitation:
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
4-10 μL of a previous lot of this clone immunoprecipitated Estrogen Receptor α from 1 mg of MCF-7 RIPA lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Routinely evaluated by western blot analysis.
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Western Blot Analysis:
This lot detected ERα at a 1:2,000 dilution in MCF-7 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Target description
66 kDa
Linkage
Replaces: 05-820
Physical form
Cultured supernantant with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
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Florent Morfoisse et al.
Arteriosclerosis, thrombosis, and vascular biology, 38(6), 1346-1357 (2018-04-14)
Estrogens exert beneficial effect on the blood vascular system. However, their role on the lymphatic system has been poorly investigated. We studied the protective effect of the 17β estradiol-the most potent endogenous estrogen-in lymphedema-a lymphatic dysfunction, which results in a
Coralie Poulard et al.
EMBO molecular medicine, 4(11), 1200-1213 (2012-10-16)
Oestrogen receptors can mediate rapid activation of cytoplasmic signalling cascades by recruiting Src and PI3K. However, the involvement of this pathway in breast cancer remains poorly defined. We have previously shown that methylation of ERα is required for the formation
Coactivators enable glucocorticoid receptor recruitment to fine-tune estrogen receptor transcriptional responses.
Bolt, MJ; Stossi, F; Newberg, JY; Orjalo, A; Johansson, HE; Mancini, MA
Nucleic Acids Research null
Victoria L Nutsch et al.
Hormones and behavior, 96, 4-12 (2017-09-09)
Testosterone is the main circulating steroid hormone in males, and acts to facilitate sexual behavior via both reduction to dihydrotestosterone (DHT) and aromatization to estradiol. The mPOA is a key site involved in mediating actions of androgens and estrogens in
Defining estrogenic mechanisms of bisphenol A analogs through high throughput microscopy-based contextual assays.
Stossi, F; Bolt, MJ; Ashcroft, FJ; Lamerdin, JE; Melnick, JS; Powell, RT; Dandekar et al.
Chemistry & Biology null
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