ZRB1964

Anti-MLH1 Antibody, clone 1B15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): DNA mismatch repair protein Mlh1;MutL protein homolog 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B15, recombinant monoclonal
• description: 1B15 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 84.6 kDa; observed mol wt ~80 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_000240
• UniProt accession no.: P40692
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MLH1(4292)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1B15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects MLH1. It targets an epitope within 22 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the C-terminal half of human DNA mismatch repair protein MLH1.

Application

Quality Control Testing

Evaluated by Western Blotting in MOLT4 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MLH1 in MOLT4 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected MLH1 in U2OS cell lysate.

Affinity Binding Assay: A representative lot of this antibody bound MLH1 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MLH1 in human colon tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MLH1 in U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in MOLT4 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected MLH1 in MOLT4 cell lysate.

Target description

DNA mismatch repair protein Mlh1 (UniProt: P40692; also known as MutL protein homolog 1) is encoded by the MLH1 (also known as COCA2) gene (Gene ID: 4292) in human. DNA mismatch repair (MMR) is an evolutionarily conserved process that corrects DNA polymerase errors during replication to maintain genomic integrity. MLH1 is a component of the DNA MMR complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. It can form heterodimers with PMS2, PMS1, or MLH3. These heterodimers bind to mismatches and insertions in DNA, as well as certain types of DNA adducts that are induced by drugs, such as cisplatin or monofunctional alkylating agents. The attachment of the MLH1/PMS2 complex is reported to cause degradation of the mutated DNA sequence fragment and the restart of synthesis. MLH1 expression is observed in colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder, and heart tissue. It plays a key role in correcting mismatches in DNA occurring during DNA replication and have been implicated in the engagement of apoptosis induced by a number of cytotoxic anticancer agents. Mutations in MLH1 gene have been linked to familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological, and female reproductive tracts. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Traver, S., et al. (2015). Mol. Cell. 59(5); 831-839; Allison, AR., et al. (2004). Nucleic Acids Res. 32(18); 5321-5338).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable