T8778
Trehalase from porcine kidney
buffered aqueous glycerol solution, ≥1.0 units/mg protein
Synonym(s):
α,α-Trehalose glucohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
buffered aqueous glycerol solution
specific activity
≥1.0 units/mg protein
concentration
0.5-10.0 mg/mL protein (BCA)
storage temp.
−20°C
Related Categories
General description
Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.
Application
Trehalase has been used:
- in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
- in a study to investigate growth arrest by trehalose-6-phosphate
- to quantify the intracellular trehalose in Lactobacillus casei cells
Biochem/physiol Actions
Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.
Unit Definition
One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).
Physical form
Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5
Legal Information
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Although trehalose has recently gained interest because of its pharmaceutical potential, its clinical use is hampered due to its low bioavailability. Hence, hydrolysis-resistant trehalose analogues retaining biological activity could be of interest. In this study, 34 4- and 6-O-substituted trehalose
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