T4069

JC-1

solid

• Synonym(s): 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3)
• Empirical Formula (Hill Notation): C₂₅H₂₇Cl₄IN₄
• CAS Number: 3520-43-2
• Molecular Weight: 652.23
• MDL number: MFCD00188475
• UNSPSC Code: 12171500
• PubChem Substance ID: 24900193
• NACRES: NA.47

Properties

• Assay: ≥97%
• Quality Level: 100
• form: solid
• color: red
• ε (extinction coefficient): ≥175000 at 511-517 nm in methanol
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [I-].CCN1C(=C\C=C\c2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)/N(CC)c4cc(Cl)c(Cl)cc14
• InChI: 1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
• InChI key: FYNNIUVBDKICAX-UHFFFAOYSA-M

Description

Application

The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.

A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λ_ex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λ_em 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves