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Key Documents

T3195

Sigma-Aldrich

Anti-γ-Tubulin Antibody

rabbit polyclonal

Synonym(s):

Anti-CDCBM4, Anti-GCP-1, Anti-TUBG, Anti-TUBGCP1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.43

product name

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 48 kDa

species reactivity

chicken, human

General description

Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins.

Immunogen

synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH.

Application

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in:
  • western blotting
  • immunocytochemistry
  • immunofluorescence

Biochem/physiol Actions

The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Microtubule-organizing centres: a re-evaluation
Luders J and Stearns T
Nature Reviews in Molecular and Cell Biology, 8(2), 161-161 (2007)
Nihal Kaplan et al.
The ocular surface, 21, 193-205 (2021-06-14)
To understand the relationship between ciliogenesis and autophagy in the corneal epithelium. siRNAs for EphA2 or PLD1 were used to inhibit protein expression in vitro. Morpholino-anti-EphA2 was used to knockdown EphA2 in Xenopus skin. An EphA2 knockout mouse was used
Yongxia Zhu et al.
Cancer chemotherapy and pharmacology, 86(1), 33-43 (2020-06-11)
Polo-like kinase 4 (PLK4), a member of the polo-like kinase family, plays several important roles in mitotic regulation, including centrosome duplication, spindle formation, and cytokinesis. PLK4 overexpression is frequently detected in many human cancers, including ovarian cancer, and the inhibition
The centrosomal kinase Aurora-A/STK15 interacts with a putative tumor suppressor NM23-H1
Du J and Hannon GJ
Nucleic Acids Research, 30(24), 5465-5475 (2002)
Jian Du et al.
Nucleic acids research, 30(24), 5465-5475 (2002-12-20)
Alterations in the activity of the centrosomal kinase, Aurora-A/STK15, have been implicated in centrosome amplification, genome instability and cellular transformation. How STK15 participates in all of these processes remains largely mysterious. The activity of STK15 is regulated by phosphorylation and

Articles

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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