T1949
Total Protein Reagent
Synonym(s):
Biuret Reagent
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About This Item
UNSPSC Code:
12352202
NACRES:
NC.07
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Quality Level
Application
Total Protein Reagent can be used for the quantitative, colorimetric determination of total protein concentration in solution at 540 nm. The total protein reagent is supplied as a ready-to-use liquid.
Total Protein Reagent has been used in the biuret assay.
Biochem/physiol Actions
A biuret-based total protein reagent. Copper ions in the alkaline reagent react with peptide bonds in proteins resulting in a purple color with an absorbance maximum of 540 nm.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Determination of serum proteins by means of the biuret reagent.
Gornall, A. G., et al.,
The Journal of Biological Chemistry, 177, 759-759 (1949)
B T Doumas et al.
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We developed a candidate Reference Method for measuring total serum protein by use of the biuret reaction. The method involves a previously described biuret reagent (Clin. Chem. 21: 1159, 1975) and Standard Reference Material (SRM) 927 bovine albumin (National Bureau
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