T1378

Sigma 7-9^®

≥99% (titration), crystalline

• Synonym(s): Trizma^® base, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 24899961
• NACRES: NA.25

Properties

• description: aminopeptidase substrate
• Quality Level: 200
• Assay: ≥99% (titration)
• form: crystalline
• storage condition: dry at room temperature
• technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable
• impurities: ≤0.5% water (Karl Fischer)
• color: white to off-white
• pH: 10.5-12
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: 0.667 g/mL, clear, colorless to faintly yellow
• absorption: ≤0.4 at 290 at 40%
• application(s): clinical research; diagnostic assay manufacturing; life science and biopharma; sample preparation
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Sigma 7-9^® also known as Trizma base or tris(hydroxymethyl)aminomethane, is a versatile biological buffer used in various applications, such as protein freezing, electrophoresis, and the preparation of buffer solutions such as TAE and TBE. With a pKa of 8.06, Tris is particularly valuable in labs working within the typical physiological pH range of living organisms (pH 7.0-9.0). It is also commonly employed in combination with other buffering agents to precisely adjust solution pH.

Additionally, Tris aids in enhancing the permeability of cell membranes. This compound is indispensable in laboratories where it is utilized to prepare buffers compatible with biological fluids, serve as a standard pH solution, and facilitate procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells.

Application

Sigma 7-9^® has been used:

• as a buffer for sample preparation in a study involving Enzyme-linked immunosorbent assay (ELISA)
• as a buffer for sample preparation in research involving immunohistochemistry and immunofluorescence

Features and Benefits

• Suitable for Biological and Biochemical Research
• Effective Buffering from pH 7-9 (25 °C) with a pKa of 8.1 (25 °C)

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

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Legal Information

Sigma 7-9 is a registered trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves