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SAB4200606

Sigma-Aldrich

Anti-EB3 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-EBF3, Anti-EBF3-S, Anti-MAPRE3, Anti-RP/EB family, Anti-RP3, Anti-member 3, Anti-microtubule-associated protein

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~32 kDa

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction)
immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAPRE3(22924)

General description

End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL.

Immunogen

Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3.

Application

Anti-EB3 antibody produced in rabbit has been used in:
  • immunoblotting
  • co-immunoprecipitation
  • immunocytochemistry
  • glutathione S-transferase (GST) pull-down assay

Biochem/physiol Actions

In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stim2-Eb3 association and morphology of dendritic spines in hippocampal neurons
Pchitskaya E, et al.
Scientific reports, 7(1), 17625-17625 (2017)
Plus-end-tracking proteins and their interactions at microtubule ends
Galjart N
Current Biology, 20(12), R528-R537 (2010)
PSD-95 alters microtubule dynamics via an association with EB3
Sweet ES, et al.
The Journal of Neuroscience, 31(3), 1038-1047 (2011)
EB3 regulates microtubule dynamics at the cell cortex and is required for myoblast elongation and fusion
Straube and Merdes A
Current Biology, 17(15), 1318-1325 (2007)
End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment
Leterrier C, et al.
Proceedings of the National Academy of Sciences of the USA, 108(21), 8826-8831 (2011)

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