SAB3501209

Anti-West Nile Virus Core antibody produced in rabbit

affinity isolated antibody

• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: liquid
• species reactivity: West Nile virus
• concentration: 1 mg/mL
• technique(s): ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_776011
• UniProt accession no.: P06935
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

West Nile Virus Core Antibody: West Nile Virus (WNV) is a member of the Flaviviridae, a plus-stranded virus family that includes St. Louis encephalitis virus, yellow fever virus, and Dengue virus. WNV was initially isolated in 1937 in the West Nile region of Uganda and has become prevalent in Africa, Asia, and Europe. It has rapidly spread across the United States with cases being observed in every continental state. Virus particles consist of a dense core made up of the core/capsid protein encapsulating the RNA genome surrounded by a membrane envelope embedded with envelope and matrix proteins which play a major role for WNV entry into target cells. The viral core protein is thought to contribute to the WNV-associated inflammation via apoptosis induced though the caspase-9 pathway as delivery of core gene delivery into the striatum of mouse brain and skeletal muscle resulted in cell death and inflammation.

Immunogen

West Nile Virus Core antibody was raised against a synthetic peptide corresponding to 15 amino acids near the carboxy terminus of the West Nile Virus core protein.The immunogen is located within amino acids 80 - 130 of West Nile Virus Core.

Application

West Nile virus Core antibody can be used for the detection of the WNV Core protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL. This antibody will also detect the protein in immunoblot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. Antibody validated: Western Blot in human samples and Immunocytochemistry in monkey samples. All other applications and species not yet tested.

Physical form

Supplied in PBS containing 0.02% sodium azide.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable