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Key Documents

SAB2702219

Sigma-Aldrich

Monoclonal Anti-6X His tag antibody produced in mouse

clone GT359, affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT359, monoclonal

form

buffered aqueous solution

concentration

0.4mg/mL

technique(s)

western blot: 1:1000-1:20000

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

General description

6X His is a histidine tag that consists of a series of histidine residues at the amino or carboxy terminus of a protein.

Immunogen

The immunogen used to generate this antibody corresponds to 6X His tag

Application

Suggested starting dilutions are as follows: WB: 1:1000-1:20000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

6X His tag does not affect the structure and function of the protein and can be used in antibody production.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

0.05M Tris, 0.15M NaCl (pH7.4). 0.025% ProClin 300 was added as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H317 - H411

Hazard Classifications

Aquatic Chronic 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Molecular Biology of Streptococci (2007)
The Dictionary of Genomics, Transcriptomics and Proteomics (2015)
Abhishek Kumar et al.
Journal of cell science, 133(14) (2020-06-28)
Mitochondrial biogenesis requires efficient sorting of various proteins into different mitochondrial sub-compartments, mediated by dedicated protein machinery present in the outer and inner membrane. Among them, the TIM22 complex enables the integration of complex membrane proteins with internal targeting signals
Yajun Xie et al.
Nature communications, 10(1), 4266-4266 (2019-09-21)
Decreased expression of 4-hydroxyphenylpyruvic acid dioxygenase (HPD), a key enzyme for tyrosine metabolism, is a cause of human tyrosinemia. However, the regulation of HPD expression remains largely unknown. Here, we demonstrate that molecular chaperone TTC36, which is highly expressed in

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