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RAB0352

Sigma-Aldrich

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

192 assays

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

usage

192 assays

species reactivity

mouse, human, rat

technique(s)

ELISA: suitable
capture ELISA: suitable

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MAPK1(5594) , MAPK14(1432) , MAPK3(5595) , MAPK8(5599)
mouse ... MAPK1(26413) , MAPK14(26416) , MAPK3(26417) , MAPK8(26419)
rat ... MAPK1(116590) , MAPK14(81649) , MAPK3(50689) , MAPK8(116554)

General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABBLOCK5x Blocking Solution (Item F)SDS
  • RABFIX1Fixing Solution (Item D)SDS
  • RABHRP1HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)SDS
  • RABHRP2HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)SDS
  • RABPLATE1Uncoated MicroplateSDS
  • RABQUENCHQuenching Solution for Cell-based ELISA Assay (Item E)SDS
  • RABSTOP1Stop SolutionSDS
  • RABTMB1TMB Substrate Reagent (Item J)SDS
  • RABWASH120X Wash Buffer Concentrate ASDS
  • RABWASH220X Wash Buffer Concentrate BSDS
See All (10)

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Certificates of Analysis (COA)

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Xiaoqing Zhang et al.
Stem cell research, 32, 115-125 (2018-10-03)
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function and they are required for vascular tissue regeneration. Multipotent adipose derived stromal cells (ASCs) can be differentiated into VSMC-like cells, which can be used as

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