R8508
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae)
buffered aqueous solution
Synonym(s):
Transfer RNA, tRNA
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About This Item
Recommended Products
grade
for molecular biology
Quality Level
form
buffered aqueous solution
concentration
9-11 mg/mL
foreign activity
DNase, Nickase, none detected
shipped in
dry ice
storage temp.
−20°C
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General description
Transfer RNA (tRNA) is isolated from baker′s yeast.
Transfer RNA (tRNA) is a short nucleotide RNA chain, which has an L-shaped structure.
Application
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used to precipitate mRNA of denuded oocytes and for reverse transcription. It has also been used to seal magnetic beads in RNA pull-down assay of nasopharyngeal carcinoma (NPC) cells.
Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) is suitable for use as a carrier in nucleic acid purification and precipitation.
Biochem/physiol Actions
Transfer RNA (tRNA) acts as an ‘adaptor′ molecule that translates the three-nucleotide codon sequence in the mRNA to the corresponding amino acid of that codon. It determines the genetic code by linking amino acids and nucleic acids. tRNA is involved in viral replication, bacterial cell wall biosynthesis, cell stress response, and animal behavior control. Aberrations in tRNA molecules are associated with several human diseases.
Components
tRNA is provided in a solution in 10 mM Tris HCl (pH 7.4) in 1 mM EDTA.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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