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O3757

Sigma-Aldrich

Octyl β-D-glucopyranoside solution

≥95% (HPLC), 50 % (w/v) in H2O

Synonym(s):

n-Octyl glucoside, OGP

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About This Item

Empirical Formula (Hill Notation):
C14H28O6
CAS Number:
Molecular Weight:
292.37
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NB.22

description

non-ionic

Quality Level

Assay

≥95% (HPLC)

mol wt

average mol wt 24500-25000

concentration

50 % (w/v) in H2O

aggregation number

84

CMC

20-25

transition temp

cloud point ≥100

application(s)

detection

storage temp.

−20°C

SMILES string

CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O

InChI

1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1

InChI key

HEGSGKPQLMEBJL-RKQHYHRCSA-N

Application

2.5% n-octyl β-D glucopyranoside has been used to homogenize mouse retina
n-Octyl β-D-glucopyranoside (OGP) is a non-ionic detergent which has been used for isoelectric focusing (IEF) and two-dimensional electrophoresis (2D). OGP has been shown to be superior to Triton X-100 for IEF of plant proteins. n-Octyl β-D-glucopyranoside has also been used for concentration of protein from 2D gels for digestion and mass spectroscopy analysis.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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M F Lopez
Journal of chromatography. B, Biomedical sciences and applications, 722(1-2), 191-202 (1999-03-06)
Two-dimensional electrophoresis has rapidly become the method of choice for resolving complex mixtures of proteins. Since the technique was pioneered in 1975, 2-D gel methods have undergone a series of enhancements to optimize resolution and reproducibility. Recent improvements in the
P J Holloway et al.
Analytical biochemistry, 172(1), 8-15 (1988-07-01)
A technique for the analysis of plant proteins from seed, leaf, root, and coleoptile tissues by high resolution two-dimensional electrophoresis is described. This technique is based primarily on the procedure of P. O'Farrell (1975, J. Biol. Chem. 250, 4007-4021); however
Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination.
Song H, et al.
The Journal of Biological Chemistry (2007)
P Dainese Hatt et al.
European journal of biochemistry, 246(2), 336-343 (1997-06-01)
We have developed a gel electrophoresis system that can concentrate proteins from spots cut out of up to 50 two-dimensional electrophoresis gels. During protein concentration, SDS is substituted with a non-ionic detergent (octyl beta-glucopyranoside) which allows digestion and MS analysis
Christine Ménager et al.
Langmuir : the ACS journal of surfaces and colloids, 26(19), 15453-15463 (2010-09-10)
The present study deals with the morphological modifications of giant dioleoyl phosphatidylcholine vesicles (DOPC GUVs) induced by the nonionic surfactant n-octyl β,D-glucopyranoside at sublytic levels, i.e., in the first steps of the vesicle-to-micelle transition process, when surfactant inserts into the

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