N3262
NCTC 135 Medium
With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
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About This Item
UNSPSC Code:
12171500
NACRES:
NA.75
Recommended Products
form
powder
technique(s)
cell culture | mammalian: suitable
components
NaHCO3: no
L-glutamine: 0.13573 g/L
glucose: 1.0 g/L
phenol red: 0.02 g/L
shipped in
ambient
storage temp.
2-8°C
General description
NCTC 135 Medium is ideal for serum-free growth of mouse L-cells.
Application
NCTC 135 Medium has been used:
- to culture Philophthalmus sp. rediae in vitro
- to culture Ehrlich ascites mouse tumor cell line
- as a culture media for the differentiation of Rcho-1 cells
Quantity
Formulated to contain 9.3 grams of powder per liter of medium.
Reconstitution
Supplement with 2.2 g/L sodium bicarbonate.
supplement
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
Melanie M Lloyd et al.
Experimental parasitology, 129(2), 101-106 (2011-08-02)
The ability to culture parasites outside their host (i.e. in vitro) is essential for several aspects of parasitological research. Here, a culture medium for marine trematode parthenitae was optimized using Philophthalmus sp. rediae from the intermediate snail host, Zeacumantus subcarinatus.
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Protocols
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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