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MBD0003

Sigma-Aldrich

Microbial DNA standard from Proteus vulgaris

Suitable for PCR, sequencing and NGS

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.24

Quality Level

200

form

liquid

concentration

10 ng/μL

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

ambient

storage temp.

−20°C

General description


Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses.1 Proteus vulgaris genomic DNA microbial standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.
Proteus vulgaris is a rod-shaped gram negative, facultative anaerobe bacterium. It inhabits the intestinal tract of humans and animals and can be found in soil, water and feces.2 P. vulgaris is a member of Enterobacteriacea family that are opportunistic pathogens in humans, responsible for urinary tract and burn infections. 3 P. vulgaris was also observed fecal samples of healthy individuals. 4Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The genomic DNA is provided at ≥10 ng/μl concentration in TE buffer pH 8.0

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Proteus vulgaris urinary tract infections in rats; treatment with nitrofuran derivatives
D. J. N. Hossack
British Journal of Pharmacology and Chemotherapy, 306-312 (1962)
Nerea Porres-Osante et al.
Microbial ecology, 70(1), 132-140 (2014-12-17)
The intestinal tract is a huge reservoir of Enterobacteriaceae, some of which are opportunist pathogens. Several genera of these bacteria harbour intrinsic antibiotic resistance genes, such as ampC genes in species of Citrobacter, Enterobacter or Escherichia genera. In this work
D C Morrison et al.
Annual review of medicine, 38, 417-432 (1987-01-01)
In this chapter, current concepts about the mechanisms of action of endotoxin are reviewed. Particular attention is focused upon endotoxin-induced production of soluble mediators from macrophages and mononuclear cells and on the potential contribution of these mediators to endotoxin shock.
J Paul Brooks et al.
BMC microbiology, 15, 66-66 (2015-04-17)
Characterizing microbial communities via next-generation sequencing is subject to a number of pitfalls involving sample processing. The observed community composition can be a severe distortion of the quantities of bacteria actually present in the microbiome, hampering analysis and threatening the

Articles

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

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