MAK497

Xanthine Oxidase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• UNSPSC Code: 12352204
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: colorimetric; fluorometric
• relevant disease(s): gastrointestinal diseases; cardiovascular diseases; infectious diseases
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Xanthine Oxidase catalyzes the oxidation of xanthine to uric acid. In addition, xanthine oxidase can catalyze the oxidation of hypoxanthine to xanthine, act on certain purines and aldehydes, and in certain cases, produce the superoxide ion. Clinically, xanthine oxidase activity in blood can act as a marker for influenza, liver damage, and possibly cardiovascular health.

Features and Benefits

• Linear detection range: 0.03 to 25 U/L xanthine oxidase for the colorimetric assay and 0.01 to 2.5 U/L for the fluorometric assay.

Suitability

The kit is suitable for xanthine oxidase activity determination in cell lysate, serum, and other biological samples, as well as for studying the effects of drugs on xanthine oxidase metabolism.

Principle

The Xanthine Oxidase Assay Kit uses a single working reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to xanthine oxidase activity in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2