MAK316

Heme Assay Kit

sufficient for 250 colorimetric tests

• Synonym(s): Heme Quantification Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• detection method: colorimetric
• relevant disease(s): hematological disorder
• storage temp.: 2-8°C

Description

General description

Heme is a member of the porphyrin family. It is synthesized in both mitochondria and cytoplasm, and is a key prosthetic group for various essential proteins such as hemoglobin, cytochromes, catalases and peroxidases. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Heme determination is widely practiced by researchers of various blood diseases.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the quantitative determination of heme and evaluation of drug effects on heme metabolism in a variety of biological samples such as blood, plasma, serum, urine, and heme carrying enzymes.

Principle

Heme assay is based on an aqueous alkaline solution method, in which the heme is converted into a uniform colored form, producing a colorimetric (400 nm) result, directly proportional to the heme concentration in the sample. The optimized formulation reduces interference and exhibits high sensitivity. One mg/dL heme equals 15.3M, 0.001%, or 10ppm. The kit has a linear detection range of 0.6–125M heme in a 96 well format.

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Warning
• Hazard Statements: H290,H319,H410
• Precautionary Statements: P234 - P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable