L8146
Lectin from Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
Synonym(s):
Ulex europaeus agglutinin, UEA-I
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
Ulex europaeus
Quality Level
conjugate
peroxidase conjugate
Assay
70.00-90.00% (Lowry)
form
lyophilized powder
potency
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
composition
Protein, ~80% Lowry
technique(s)
western blot: suitable
storage temp.
2-8°C
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Application
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Biochem/physiol Actions
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Packaging
Package size based on protein content.
Linkage
Conjugates are prepared from affinity purified lectin.
Unit Definition
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Physical form
Contains sodium citrate buffer salts
Preparation Note
Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.
Repurified by affinity chromatography after conjugation.
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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