I7633
Iscove′s Modified Dulbecco′s Medium
With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
Synonym(s):
Iscoves MDM, IMDM
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About This Item
form
powder
technique(s)
cell culture | hybridoma: suitable
cell culture | mammalian: suitable
components
phenol red: yes
glucose: yes
L-glutamine: yes
shipped in
ambient
storage temp.
2-8°C
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General description
Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Application
Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.
Quantity
Formulated to contain 17.7 grams of powder per liter of medium.
Reconstitution
Supplement with 3.024 g/L sodium bicarbonate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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