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I2393

Sigma-Aldrich

Interleukin-1β from rat

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IL-1β, Interleukin-1β from rat, Lymphocyte activating factor

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About This Item

MDL number:
MFCD01094098
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

rat

Quality Level

100

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

0.01-0.1 ng/mL ED50/EC50

mol wt

17.3 kDa

packaging

pkg of 10 and 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg

UniProt accession no.

Q63264

storage temp.

−20°C

Gene Information

rat ... Il1b(24494)

Related Categories

General description

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.1 IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,2 and is thought to play a key role in inflammatory and immune responses.3 Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFH), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).4 The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Analysis Note

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Citation

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Wei Zhou et al.
Journal of neurophysiology, 117(6), 2282-2291 (2017-03-17)
Excessive inflammation including IL-1β-initiated signaling is among the earlies reactions that can cause neuronal damage following spinal cord injury (SCI). It has been suggested that microRNAs may participate in stem cell repair to facilitate functional recovery following SCI. In this

Articles

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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