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InChI
1S/C24H42O21/c25-1-5-9(28)11(30)16(35)22(41-5)39-4-8-20(45-23-17(36)12(31)10(29)6(2-26)42-23)14(33)18(37)24(43-8)44-19-7(3-27)40-21(38)15(34)13(19)32/h5-38H,1-4H2/t5-,6-,7-,8-,9-,10-,11+,12+,13-,14-,15-,16-,17-,18-,19-,20-,21+,22+,23-,24-/m1/s1
InChI key
BYSGBSNPRWKUQH-UJDJLXLFSA-N
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General description
Glycogen is a branched polymer of glucose synthesized by animal cells for energy storage and release. It is constructed of predominantly α1→4 glycosidic bonds with branches created through α1→6 glycosidic bonds.
Glycogen, a polysaccharide of glucose, serves as the primary storage of glucose in the body and functions as a vital energy reserve in both fungi and animals. With its multibranched structure, glycogen is a secondary long-term energy storage molecule, similar to starch, and closely related to amylopectin, playing a pivotal role in energy storage and release and existing as granules in the cytoplasm.
In research, glycogen is commonly employed to investigate carbohydrate storage, metabolism, and enzyme characterization. Serving as an inert carrier, it significantly enhances the efficiency of DNA and RNA extraction through ethanol precipitation. Its versatility proves valuable in unraveling the intricate details of the glucose cycle, contributing to advancements in diverse areas of physiological, nutritional, biochemical, and cell biology research.
In research, glycogen is commonly employed to investigate carbohydrate storage, metabolism, and enzyme characterization. Serving as an inert carrier, it significantly enhances the efficiency of DNA and RNA extraction through ethanol precipitation. Its versatility proves valuable in unraveling the intricate details of the glucose cycle, contributing to advancements in diverse areas of physiological, nutritional, biochemical, and cell biology research.
The Pacific oyster/Crassostrea gigas is a vital marine fishery resource. 20-40% of the dry weight of an oyster accounts for glycogen. High levels of glycogen contribute to the flavor, quality, and hardiness of the oyster.
Application
Glycogen from oyster has been used:
- as a component in acrylamide slab gels to perform branching enzyme activity staining for quantifying ADP-glucose pyrophosphorylase (AGPase) activity
- as a sterile inflammatory agent for inoculating into mice for obtaining peritoneal exudate cells (PEC) and to study its effects on the metabolic functions of macrophages
- as a primer for supplementing maleate buffer for amylosucrase assay to measure the activities of the glutathione S-transferase (GST-AS) amylosucrase fusion protein and the purification fractions
- as a supplement in PreScission buffer to perform polymer synthesis reaction
Oyster glycogen has been used to induce inflammation and to promote extravasation of granulocytes and neutrophils in animal models after intraperitoneal injection. Glycogen also acts as a vascular permeability probe.
Features and Benefits
- Ideal for Biochemical and Cell Biology research
- Versatile and adaptable for wide variety of laboratory and research applications
Other Notes
For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Henrik U Irgens et al.
The Journal of clinical endocrinology and metabolism, 100(5), E767-E775 (2015-03-10)
The synthesis of glycogen is initiated by glycogenin. In humans, glycogenin-1 is expressed ubiquitously, whereas glycogenin-2 (GN2) is highly expressed in liver. It has therefore been suggested that GN2 is a liver isoform of glycogenin. In a search for possible
A M Kaplan et al.
The Journal of experimental medicine, 146(5), 1461-1466 (1977-11-01)
A macrophage cell-surface antigen associated with pyran and Corynebacterium parvum-activated macrophages and P388D1 cells but not detectable on normal or glycogen and thioglycollate-elicited murine macrophages has been described. The antigen was demonstrated both by complement-mediated cytotoxicity and immunofluorescence, using an
Naoko Fujita et al.
Journal of experimental botany, 60(3), 1009-1023 (2009-02-05)
Rice (Oryza sativa) allelic sugary1 (sug1) mutants defective in isoamylase 1 (ISA1) accumulate varying levels of starch and phytoglycogen in their endosperm, and the activity of a pullulanase-type of a debranching enzyme (PUL) was found to correlate closely with the
Naoko Fujita et al.
Journal of experimental botany, 62(14), 4819-4831 (2011-07-07)
Starch synthase (SS) I and IIIa are the first and second largest components of total soluble SS activity, respectively, in developing japonica rice (Oryza sativa L.) endosperm. To elucidate the distinct and overlapping functions of these enzymes, double mutants were
Busu Li et al.
BMC genomics, 18(1), 713-713 (2017-09-13)
The Pacific oyster Crassostrea gigas is an important marine fishery resource, which contains high levels of glycogen that contributes to the flavor and the quality of the oyster. However, little is known about the molecular and chemical mechanisms underlying glycogen
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