DN25
Deoxyribonuclease I from bovine pancreas
lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein
Synonym(s):
Deoxyribonuclease, DNase I, Deoxyribonucleate 5′-oligonucleotido-hydrolase
About This Item
Recommended Products
biological source
bovine pancreas
Quality Level
form
lyophilized powder
specific activity
≥400 Kunitz units/mg protein
mol wt
~31 kDa
composition
Protein, ≥85%
technique(s)
DNA extraction: suitable
solubility
0.15 M NaCl: soluble 5.0 mg/mL, hazy
suitability
suitable for molecular biology
application(s)
diagnostic assay manufacturing
diagnostic assay manufacturing
foreign activity
RNase ≤0.02%
shipped in
wet ice
storage temp.
−20°C
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General description
- Our DNase I Digests double str and single stranded DNA into oligo and mononucleotides.
- Bovine pancreatic DNase exists as four isozymes, having isoelectric points for A, B, C and D: 5.22, 4.96, 5.06 and 4.78.3. The predominant form is A, with smaller amounts of B and C, and only minor amount of D.
- DNase I structure resembles the structure of to exonuclease III. It includes two central ß sheets. Each β sheet is composed of six β-strands. This complex of β sheets is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III.[1]
Application
- Decreases viscosity providing better yields by removing DNA in primary cell isolation:
- Incorporating labelled bases into DNA: DNA nick
- Radioactive labelling
- Bioprocessing applications: DNA removal
- Eliminating genomic DNA from RNA preparations before RT-PCR
- In vitro transcription
- Nick translation
- DNase footprinting
- Actin regulation of actin polymerization in cells, and cell apoptosis
- UV crosslinking of proteins to nucleic acids
- DNase play a role in the regulation of actin polymerization in cells and is involved in apoptosis process [1]
Biochem/physiol Actions
Features and Benefits
Unit Definition
Physical form
Preparation Note
Analysis Note
inhibitor
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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To standardize a procedure for the enzymatic assay of Deoxyribonuclease I at Sigma-Aldrich St. Louis.
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