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D0547

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

DME/F-12, 1:1 mixture

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.71

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
sodium pyruvate: 0.11 g/L
HEPES: no
phenol red: yes
glucose: 3.15 g/L
L-glutamine: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:

  • to culture cerebral cortices from mice embryos
  • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants
  • to culture human corneal epithelial cell lines

Other Notes

Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate

Quantity

Formulated to contain 12.0 grams of powder per liter of medium.

Reconstitution

Supplement with 1.2 g/L sodium bicarbonate

Certificates of Analysis (COA)

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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