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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
P3X63Ag8.653
Reactivity
Anti-TNF, bovine
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse P3X63Ag8.653 x Mouse BALB/c spleen
Cell Line Description
The monoclonal antibody BC9 detects bovine tumour necrosis factor (TNF) in a radioimmunoassay with a detection limit of 24 pg/ml. Bovine recombinant TNF can be neutralised in vitro by addition of BC9. Cytotoxic assays showed a neutralising effect using L929 and WEHI 164 clone 13 cells.
Immunogen
Bovine recombinant TNF-alpha
Application
Detection of TNF in bioassay or radioimmunoassay, in vivo studies (neutralisation of TNF).
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Maintain cultures between 5-6x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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