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biological source
mouse breast
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
A-type and C-type virus particles, plasminogen activator (tPA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse BALB/c mammary alveolar nodules, pre-neoplastic
Cell Line Description
Derived from pre-malignant mammary tissue of a BALB/c mouse. Produce significant amounts of C-type and A-type virus particles and low amounts of plasminogen activator.
Application
Non malignant mammary cell studies.
Culture Medium
DMEM + 2mM Glutamine + 10μg/ml Insulin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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