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biological source
Human x Mouse Hybridoma hybrid
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human (WI-L2-729-FF2) x mouse (P3X63/Ag8.653) heterohybridoma
Cell Line Description
Generated by electro-acoustic fusion of 2 lines. Parent: WI-L2-729-HF2. Murine myeloma: P3X63/Ag8.653. Non-immunoglobulin secreting, HAT-sensitive, resistant to ouabain at greater than 100uM, will fuse with normal human lymphocytes and lymphoblastoids.
Application
Fusion partner
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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