C9830
Chitosanase from Streptomyces griseus
lyophilized powder, >50 units/mg protein (Bradford)
Synonym(s):
Chitosan N-acetylglucosaminohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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form
lyophilized powder
Quality Level
specific activity
>50 units/mg protein (Bradford)
purified by
chromatography
solubility
H2O: soluble, clear to slightly hazy, colorless (10U/0.3mL)
foreign activity
chitinase <1.0 units/mg protein
storage temp.
−20°C
General description
Chitosanase is synthesized from Streptomyces griseus as an extracellular secreted enzyme and corresponds to a molecular weight of 34 kDa. It has a temperature and pH optimum of 60°C and 5.7, respectively. Chitosanase belongs to the glycosyl hydrolase family and comprises α-helical tertiary structure similar to lysozyme with substrate-binding cleft placed between two lobes.
Application
Chitosanase from Streptomyces griseus has been used in a study to assess the effect of chitin sources on production of chitinase and chitosanase. Chitosanase from Streptomyces griseus has also been used in a study to investigate the effective production of chitinase and chitosanase by Streptomyces griseus HUT 6037 using colloidal chitin and various degrees of deacetylation of chitosan.
Chitosanase from Streptomyces griseus has been used in the digestion of lacrimal gland (LG) tissues.
The enzyme from Sigma has been used for the hydrolysis of the crude cell wall preparation of oomycete Aphanomyces euteiches, a major parasite of legume plants. It has also been used for the enzymatic hydrolysis of the fully de-N-acetylated chitosan to get chitosan oligomer mixtures during the preparation of biocompatible chitosan-alginate gel.
Biochem/physiol Actions
Chitosanase catalyzes the endohydrolysis of N-acetylated chitosan specifically at the of β(1,4) linkages between N-acetyl-D-glucosamine and D-glucosamine residues. Chitosanase from Streptomyces griseus is capable of hydrolyzing both chitosan and carboxymethyl cellulose. Chitosanase is found in several types of microorganisms and is used for the lysis of fungal cell walls.
Unit Definition
One unit will release 1 μmole of glucosamine from chitosan per min at pH 5.0 at 37 °C as measured in the fluorimetric assay of Osswald, et al. (1992).
Physical form
Lyophilized powder containing potassium phosphate buffer salts
Preparation Note
For long term solution storage, reconstitute vial with 50% glycerol solution. Dilute to working concentrations with a 0.1% BSA solution.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Ilham Badreddine et al.
Eukaryotic cell, 7(11), 1980-1993 (2008-09-23)
Chitin is an essential component of fungal cell walls, where it forms a crystalline scaffold, and chitooligosaccharides derived from it are signaling molecules recognized by the hosts of pathogenic fungi. Oomycetes are cellulosic fungus-like microorganisms which most often lack chitin
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Biocompatible hydrogels are very interesting for applications in, e.g., tissue engineering and for immobilization of cells, such as calcium-alginate gels where the calcium ions form specific interactions with the guluronic acid units. We here report on a new gelling system
Chitosanase from Streptomyces griseus.
A Ohtakara
Methods in enzymology, 161, 505-510 (1988-01-01)
Toshiaki Tanabe et al.
Bioscience, biotechnology, and biochemistry, 67(2), 354-364 (2003-05-06)
Streptomyces griseus HUT 6037 inducibly produced two chitosanases when grown on chitosan. To elucidate the mechanism of degradation of chitinous compound by this strain, chitosanases I and II of S. griseus HUT 6037 were purified and characterized. The purified enzymes
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Articles
Enzymatic Assay of Chitosanase from Streptomyces Species
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