B3279

N_α-Benzoyl-L-arginine 4-nitroanilide hydrochloride

>98% (TLC)

• Synonym(s): L-BAPA, L-BAPNA, BANI
• Empirical Formula (Hill Notation): C₁₉H₂₂N₆O₄ · HCl
• CAS Number: 21653-40-7
• Molecular Weight: 434.88
• Beilstein: 4081878
• EC Number: 244-505-6
• MDL number: MFCD00063682
• UNSPSC Code: 12352204
• PubChem Substance ID: 24891697
• NACRES: NA.32

Properties

• Quality Level: 200
• Assay: >98% (TLC)
• form: powder
• solubility: acetone: water (1:1): 40.00-51.00 mg/mL, clear, colorless to yellow
• storage temp.: −20°C
• SMILES string: Cl[H].NC(=N)NCCC[C@H](NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H/t16-;/m0./s1
• InChI key: DEOKFPFLXFNAON-NTISSMGPSA-N

Description

General description

Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride (L-BAPNA) is a colorless, chromogenic substrate for proteolytic enzymes such as trypsin, actinidin, and papain.

Application

Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride has been used as substrate:

• to measure papain activity in spectrophotometric amidase assay
• to measure the inhibitory function of human cystatin C (HCC) on the protease activity of papain by colorimetric assay
• for trypsin

Substrates

Chromogenic substrate for trypsin and other proteolytic enzymes.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves