A7511
Bovine Serum Albumin
lyophilized powder, essentially fatty acid free, ≥97% (agarose gel electrophoresis), 1X Crystallized
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
biological source
bovine
Assay
≥97% (agarose gel electrophoresis)
form
lyophilized powder
quality
crystallized
mol wt
~66 kDa
purified by
cold ethanol fractionation
packaging
poly bottle of
origin
USA origin
Fatty acid content
≤0.01%
technique(s)
ELISA: suitable
electrophoresis: suitable
western blot: suitable
impurities
≤0.01% free fatty acid
pH
7
solubility
water: soluble (50 mg/ml)
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Essentially fatty acid free
- Crystallized
Preparation Note
Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method. Further purified by a proprietary crystallization method
Prepared from albumin (A4378)
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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