A2554

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Goat Anti-Mouse IgG (Fc specific)–HRP
• MDL number: MFCD00162629
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• should not react with: bovine, human, horse
• technique(s): direct ELISA: 1:70,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot (chemiluminescent): 1:80,000-1:160,000 using total cell extract of Hela cells (5-10 μg/mL)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Specificity

Goat anti-mouse IgG(Fc specific)-peroxidase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The conjugate shows no reactivity with mouse Fab fragment, human IgG, IgA, IgM, κ and λ light chain, bovine IgG and IgM, or horse IgG.

Application

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in:

• immunohistochemistry
• enzyme-linked immunosorbent assay (ELISA)
• immunoblot
• dot-blot analysis

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

Biochem/physiol Actions

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization and antibody dependent cell mediated cytotoxicity.

Other Notes

Antibody adsorbed with bovine, equine and human serum proteins.

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Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Adsorbed to reduce background staining with bovine, horse, or human samples.

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable