93145

1,3,5-Triphenyltetrazolium formazan

≥90% (UV)

• Synonym(s): TTC formazan
• Empirical Formula (Hill Notation): C₁₉H₁₆N₄
• CAS Number: 531-52-2
• Molecular Weight: 300.36
• Beilstein: 753057
• EC Number: 208-509-1
• MDL number: MFCD00020647
• UNSPSC Code: 12352204
• PubChem Substance ID: 57653521
• NACRES: NA.83

Properties

• Assay: ≥90% (UV)
• form: powder
• solubility: dioxane: 50 mg/mL, clear
• SMILES string: N(\N=C(/N=N/c1ccccc1)c2ccccc2)c3ccccc3
• InChI: 1S/C19H16N4/c1-4-10-16(11-5-1)19(22-20-17-12-6-2-7-13-17)23-21-18-14-8-3-9-15-18/h1-15,20H/b22-19-,23-21+
• InChI key: BEIHVSJTPTXQGB-YMGPPEANSA-N

Description

General description

1,3,5-Triphenyltetrazolium formazan is an insoluble compound produced upon the reduction of 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt, which is an indicator of cell redox potential. This reaction is irreversible. TTC formazan is brightly colored and can be quantified using a spectrophotometer.

Application

1,3,5-Triphenyltetrazolium formazan has been used:

• for the quantification of 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity in bacterial cells
• as a standard to study the optical density of formazan produced by TTC-treated milled seeds
• to stain heart tissue for infarct size assessment or undergo perfusion fixation to allow morphometric studies in addition to histology and immunohistochemistry

Other Notes

Review: Tetrazolium salts and formazans

Safety Information

• Pictograms: GHS07,GHS09
• Signal Word: Warning
• Hazard Statements: H302,H400
• Precautionary Statements: P264 - P270 - P273 - P301 + P312 - P391 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves