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Key Documents

04492

Sigma-Aldrich

Esterase Paenibacillus barcinonensis, recombinant

recombinant, expressed in E. coli, ≥10 U/mg

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About This Item

CAS Number:
Enzyme Commission number:
3.1.1.1
EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54

recombinant

expressed in E. coli

form

crystalline
crystals
powder or flakes

specific activity

≥10 U/mg

storage temp.

−20°C

Application

Esterase Paenibacillus barcinonensis, also known as EstA, may be useful in protein engineering studies. It has been engineered to increase enantioselectivity .

Biochem/physiol Actions

An esterase is a hydrolase that splits esters into acids and alcohols. The esterase catalyzes the transesterification of 1-phenylethanol.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which converts 1 μmol 4-nitrophenyl-L-acetate per minute at pH 7.5 and 30°C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rational Protein Design of Paenibacillus barcinonensis Esterase EstA for Kinetic Resolution of Tertiary Alcohols
Arnau Bassegoda, et al.
ChemCatChem, 2, 962-967 (2010)
B Sànchez-Nogué et al.
Environmental science and pollution research international, 20(5), 3480-3488 (2012-12-06)
The common sole, Solea solea (Linneus, 1758), and the Senegalese sole, Solea senegalensis (Kaup, 1858), are two important commercial species that coexist in the NW Mediterranean. In order to assess the species' ability to respond to chemical insults, a comparison
Marie C Fortin et al.
Drug metabolism and disposition: the biological fate of chemicals, 41(2), 326-331 (2012-12-12)
Studies on therapeutic drug disposition in humans have shown significant alterations as the result of pregnancy. However, it is not known whether pesticide metabolic capacity changes throughout pregnancy, which could affect exposure of the developing brain. We sought to determine
XieMei Tang et al.
Critical reviews in eukaryotic gene expression, 22(3), 179-187 (2012-11-13)
Tuberculosis remains one of the most prevalent and deadly infectious diseases, largely due to the emergence of multidrug-resistant and extensive drug-resistant Mycobacterium tuberculosis, especially the coinfection with HIV. Mycobacterium Ag85 complex (Ag85A, B, and C), with a carboxylesterase consensus sequence
Zhe-Yi Hu et al.
Analytical and bioanalytical chemistry, 405(5), 1695-1704 (2012-12-15)
Dabigatran etexilate (DABE) is an oral prodrug that is rapidly converted by esterases to dabigatran (DAB), a direct inhibitor of thrombin. To elucidate the esterase-mediated metabolic pathway of DABE, a high-performance liquid chromatography/mass spectrometry based metabolite identification and semi-quantitative estimation

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