T9159

Trichloroacetic acid

BioXtra, ≥99.0%

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• Beilstein: 970119
• EC Number: 200-927-2
• MDL number: MFCD00004177
• UNSPSC Code: 12352106
• PubChem Substance ID: 24900586
• NACRES: NB.22

Properties

• vapor density: <1 (vs air)
• Quality Level: 200
• vapor pressure: 1 mmHg ( 51 °C)
• product line: BioXtra
• Assay: ≥99.0%
• impurities: ≤0.0005% Phosphorus (P); ≤0.1% Insoluble matter
• ign. residue: ≤0.03%
• refractive index: n20/D 1.62 (lit.)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: sulfate (SO₄^2-): ≤0.05%
• cation traces: Al: ≤0.0005%; Ca: ≤0.005%; Cu: ≤0.0005%; Fe: ≤0.001%; K: ≤0.01%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• storage temp.: 2-8°C
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N

Description

General description

Trichloroacetic acid (TCA) is an important metabolic product of trichloroethylene (TCE) metabolism.

Application

Trichloroacetic acid has been used:

• for protein precipitation
• as a component in the extract solution used for the measurement of malondialdehyde and glutathione
• to precipitate the transfected and treated cells in [³H]-thymidine incorporation assay

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Biochem/physiol Actions

TCA might act as a carcinogen, targeting liver. It might be used as an acid decalcifying agent. TCA helps in nuclear staining.

Safety Information

• Pictograms: GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H314 - H410
• Precautionary Statements: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 2
• Flash Point(F): 235.4 °F - closed cup
• Flash Point(C): > 113 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves