97622

2-Mercaptoethanol

for HPLC derivatization, LiChropur^™, ≥99.0% (GC)

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• EC Number: 200-464-6
• MDL number: MFCD00004890
• UNSPSC Code: 12000000
• PubChem Substance ID: 329770550
• NACRES: NA.05

Properties

• grade: for HPLC derivatization
• Quality Level: 100
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• Assay: ≥99.0% (GC)
• form: liquid
• quality: LiChropur^™
• expl. lim.: 18 %
• concentration: 14.3 M (pure liquid)
• technique(s): HPLC: suitable
• refractive index: n20/D 1.500 (lit.); n20/D 1.500-1.502
• bp: 157 °C (lit.)
• density: 1.114 g/mL at 25 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

suitable for derivatisation of Amine groups

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331 - H310 - H315 - H317 - H318 - H361d - H373 - H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 165.2 °F - closed cup
• Flash Point(C): 74 °C - closed cup