11734334001

Universal Protease Substrate

lyophilized, suitable for detection, pkg of 40 mg (40 μmol; 100mM)

• Synonym(s): Substrate of protease, casein, resorufin-labeled, protease substrate, universal
• UNSPSC Code: 12352204

Properties

• form: lyophilized
• Quality Level: 100
• packaging: pkg of 40 mg (40 μmol; 100mM)
• manufacturer/tradename: Roche
• concentration: 1 mg/mL
• application(s): detection
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

The Universal Protease Substrate provides rapid and highly sensitive detection of trace protease activity, or determination of the effectiveness of protease inhibition. Protease activity releases resorufin-labeled peptides from the patented substrate, resorufin-labeled casein. Resorufin is N-(resorufin-4-carbonyl)-piperidine-4-carbonic acid).The concentration of these labeled peptides in the supernatant is directly related to the proteolytic activity present.

• Conveniently detect nanogram quantities of proteolytic activity in less than one hour.
• Perform highly sensitive protease detection in a homogeneous assay.

Application

Principle
By treatment with proteases, resorufin-labeled peptides are released from resorufin-labeled casein. They cannot be precipitated by trichloroacetic acid. The concentration of these resorufin-labeled peptides in the supernatant is equivalent to the proteolytic activity present.

Use Universal Protease Substrate as a general substrate for proteases, and to detect trace protease activities, for example, as a contamination in enzyme preparations. It can be measured spectrophotometrically and fluorimetrically in a homogeneous assay.

Quality

Function test: performance-controlled in the assay for proteases.

Preparation Note

Working concentration: Approximately 1 mg/ml
Storage conditions (working solution): -15 to -25 °C
An aqueous solution is stable for several months at -15 to -25° C and for 2 to 3 days at 2 to 8° C. It is recommended to store aqueous solutions in aliquots at -15 to -25° C. Repeated freezing and thawing is possible. At 15 to 25 °C the product is rapidly hydrolyzed in solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable